HCG
Human chorionic gonadotropin, a heterodimeric glycoprotein hormone (alpha and beta subunits with attached carbohydrate). Acts at the LH/CG receptor. Reference standard for endocrine and receptor-binding work.
Before you begin
- • Lot-matched certificate and laboratory notebook
- • Calibrated pipettes, low-binding tubes and suitable PPE
- • Validated solvent, assay reagents and model-specific SOP
- • Appropriate positive, negative and vehicle controls
Required controls
- Vehicle-only negative control
- Receptor-null or antagonist condition
- Validated reference material
- Untreated viability control
Diluent
Sterile bacteriostatic water (0.9% benzyl alcohol) for multi-withdrawal stock, or sterile water for injection where a preservative-free stock is required by the assay.
Compound-specific: This material is dosed in International Units, not milligrams. IU is a bioactivity unit, so IU-to-mass conversion is lot-specific and must come from the certificate, never from a general conversion factor.
Reconstitution concentrations
Solvent volume sets the stock concentration. Lower volume gives a more concentrated stock and smaller working aliquots; higher volume gives a more dilute stock that is easier to measure accurately.
| Solvent added | Stock concentration | Per 0.1 mL | Per unit (U-100) |
|---|---|---|---|
| 1 mL | 5000 IU/mL | 500 IU | 50 IU |
| 2 mL | 2500 IU/mL | 250 IU | 25 IU |
| 3 mL | 1666.67 IU/mL | 166.67 IU | 16.67 IU |
| 5 mL | 1000 IU/mL | 100 IU | 10 IU |
| 10 mL | 500 IU/mL | 50 IU | 5 IU |