MOTS-c
A 16-residue mitochondrial-derived peptide encoded within 12S rRNA and translated in the cytoplasm. Published work links it to AMPK signalling and metabolic homeostasis.
For laboratory research use only. Material is supplied as a research reference standard and is not approved by Health Canada. Protocols below are compiled from published literature and supplier documentation; they are reference information, not medical advice. Anyone with a health condition or on medication should speak to a physician first.
Before you begin
- • Lot-matched certificate and laboratory notebook
- • Calibrated pipettes, low-binding tubes and suitable PPE
- • Validated solvent, assay reagents and model-specific SOP
- • Appropriate positive, negative and vehicle controls
Required controls
- Vehicle-only negative control
- Untreated baseline
- Validated pathway control
- Parallel viability measurement
Reported protocol
- Standard
- 5.0 mg once or twice per week
- Timing
- 1 to 2 hours prior to physical exercise
- Route
- Subcutaneous
Diluent
Sterile bacteriostatic water (0.9% benzyl alcohol) for multi-withdrawal stock, or sterile water for injection where a preservative-free stock is required by the assay.
Reconstitution concentrations
Solvent volume sets the stock concentration. Lower volume gives a more concentrated stock and smaller working aliquots; higher volume gives a more dilute stock that is easier to measure accurately. Figures differ by vial size — use the table for the size you have.
40 mg vial
| Solvent added | Stock concentration | Per 0.1 mL | Per unit (U-100) |
|---|---|---|---|
| 1 mL | 40 mg/mL | 4 mg | 400 mcg |
| 2 mL | 20 mg/mL | 2 mg | 200 mcg |
| 3 mL | 13.33 mg/mL | 1.33 mg | 133.33 mcg |
| 5 mL | 8 mg/mL | 800 mcg | 80 mcg |
10 mg vial
| Solvent added | Stock concentration | Per 0.1 mL | Per unit (U-100) |
|---|---|---|---|
| 1 mL | 10 mg/mL | 1 mg | 100 mcg |
| 2 mL | 5 mg/mL | 500 mcg | 50 mcg |
| 3 mL | 3.33 mg/mL | 333.33 mcg | 33.33 mcg |
| 5 mL | 2 mg/mL | 200 mcg | 20 mcg |