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Researcher preparing unlabeled laboratory vials with a precision pipette
Reconstitution · Laboratory protocol

Bacteriostatic Water

Sterile water containing 0.9% benzyl alcohol as a bacteriostatic preservative. A diluent, not a test article.

For laboratory research use only. Material is supplied as a research reference standard and is not approved by Health Canada. Protocols below are compiled from published literature and supplier documentation; they are reference information, not medical advice. Anyone with a health condition or on medication should speak to a physician first.

Before you begin

  • • Lot-matched certificate and laboratory notebook
  • • Calibrated pipettes, low-binding tubes and suitable PPE
  • • Validated solvent, assay reagents and model-specific SOP
  • • Appropriate positive, negative and vehicle controls

Required controls

  • Unopened container inspection
  • Solvent blank
  • Method-specific sterility control
  • Documented lot and expiry

Diluent

Supplied ready to use. Do not dilute further.

Compound-specific: Bacteriostatic water is not the same as sterile water for injection and not the same as saline. The benzyl alcohol is what permits multiple withdrawals from one container; it is also an assay-active compound at sufficient concentration. Where the method requires a preservative-free vehicle, use sterile water instead.