TB-500
Synthetic fragment of Thymosin β4 studied in tissue-repair and angiogenesis research. Lyophilized, HPLC + mass-spec verified per lot.
Read about TB-500 in the peptide library →- Purity (HPLC)
- 99.12%
- Mol. weight
- 4963.44
- Retest by
- 2027-04-25
For laboratory research use only.
Overview
TB-500 is the synthetic acetate salt of Thymosin beta-4, a 43-residue actin-sequestering peptide found in nearly all mammalian cells. In research settings it is used to study cell migration, actin cytoskeletal dynamics, and angiogenesis, and is frequently run alongside BPC-157 in comparative musculoskeletal-repair protocols. Lot XR26042603-2 assayed at 99.12% by HPLC.
Mechanism
Binds and sequesters monomeric G-actin, holding a reserve pool that buffers filament assembly and enables rapid cytoskeletal remodeling. Published work links this actin-binding activity to accelerated cell migration, endothelial tube formation, and reduced fibrotic signaling in injury models.
Research applications
- G-actin sequestration and actin-polymerization kinetics
- Endothelial cell migration and HUVEC tube-formation assays
- Angiogenesis and wound-closure models
- Comparative musculoskeletal-repair protocols with BPC-157
- Cardiac and corneal tissue-remodeling research
Reconstitution
Allow vial to reach room temperature before opening (at least 20 minutes). Reconstitute with 1.0 to 2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct the stream onto the lyophilized cake. Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
Storage & handling
Reconstituted solution is stable for 28 days at 2 to 8 C in the original vial. For longer storage, aliquot into low-binding tubes and hold at -80 C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
Selected references
- 1. Goldstein AL et al. Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421-429.
- 2. Malinda KM et al. Thymosin beta4 accelerates wound healing. J Invest Dermatol. 1999;113(3):364-368.
TB-500 on PubMed
Independent published literature indexed on PubMed (U.S. National Library of Medicine). Provided for laboratory research reference only.
- PMID 17468232beta-ThymosinsAnn N Y Acad Sci · 2007 · PubMed →
- PMID 41966639Safety and Efficacy of Approved and Unapproved Peptide Therapies for Musculoskeletal Injuries and Athletic PerformanceSports Med · 2026 · PubMed →
- PMID 30063853Thymosin beta 4 and the eye: the journey from bench to bedsideExpert Opin Biol Ther · 2018 · PubMed →
TB-500 questions
- What is TB-500?
- TB-500 is the synthetic acetate salt of Thymosin beta-4, a 43-residue actin-sequestering peptide found in nearly all mammalian cells. It is supplied as a lyophilized research reference standard.
- What purity is TB-500, and is there a COA?
- Every lot of TB-500 is independently verified by Janoshik (reverse-phase HPLC for purity, mass spec for identity) to a ≥99% purity floor. The lot-specific Certificate of Analysis is tied to the number on the vial label and available on request.
- How is TB-500 shipped in Canada?
- TB-500 ships domestically across Canada, dispatched within 24-48 hours via Canada Post Xpresspost and arriving in about four days. No border to cross, no customs. Flat $25 CAD shipping, free over $200.
- How do I reconstitute TB-500?
- Allow vial to reach room temperature before opening (at least 20 minutes). Reconstitute with 1.0 to 2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct the stream onto the lyophilized cake. Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
- How should TB-500 be stored?
- Reconstituted solution is stable for 28 days at 2 to 8 C in the original vial. For longer storage, aliquot into low-binding tubes and hold at -80 C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
- Is TB-500 legal to buy in Canada?
- TB-500 is sold as a laboratory research reference standard, for laboratory research use only. It is legal to sell for that purpose and is not approved by Health Canada as a drug.
